Stack
Methods and tools — from molecular biology to clinical imaging.
Molecular
Methods
RNAscope, Immunofluorescence
Tools
Confocal microscopy, image analysis pipelines
Mapping gene expression and protein localization in skull base synchondroses.
3D Imaging
Methods
Whole-mount LightSheet Fluorescent Microscopy
Tools
Tissue clearing protocols, Imaris, Fiji/ImageJ
Volumetric visualization of synchondrose architecture in developing mouse skulls.
Biomechanics
Methods
Ex vivo mechanical stimulation
Tools
Custom culture rigs, force measurement, analysis software
Investigating mechanical response of skull base cartilage under controlled loading.
Clinical Atlas
Methods
Human skull base atlas (fetal → end of growth)
Tools
CT/CBCT imaging, 3D Slicer, statistical shape analysis
Building a reference atlas of synchondrose morphology across development, in control and achondroplasia.